Develops and manufactures biophysical measurement instruments for protein stability and binding analysis
NanoTemper Technologies GmbH
Munich · South San FranciscoNew on Amplifa
About NanoTemper
NanoTemper Technologies develops, manufactures and distributes biophysical characterization instruments for research and biopharma production. The portfolio includes the product lines Prometheus Panta (protein stability via nanoDSF, DLS, SLS and backreflection), Dianthus and Dianthus uHTS (affinity screening with Spectral Shift and TRIC, 384-well plates) as well as Monolith Omni (affinity and kinetics measurement based on MicroScale Thermophoresis with nanoTAK up to kon 10⁸ M⁻¹s⁻¹) and Andromeda (stability measurement in crude lysates). The systems operate with sample volumes from 10 µL, without immobilization, and deliver structured, AI-ready data exports. Applications include drug discovery, biologics development, membrane protein and structural research as well as targeted protein degradation. The company was founded in Munich in 2008 and maintains a branch office in South San Francisco; additionally, IQOQ qualification, service packages, training and assay consultation are offered.
- Site
- München
- Processes
- nanoDSF (nano Differential Scanning Fluorimetry) – protein stability characterization, Dynamic Light Scattering (DLS) – hydrodynamic radius, polydispersity, aggregation, Static Light Scattering (SLS) – molecular weight determination, self-interaction, Backreflection – turbidity/aggregate measurement, MicroScale Thermophoresis (MST) – binding affinity measurement in solution, Temperature Related Intensity Change (TRIC) – fluorescence-based binding detection, Spectral Shift – affinity determination via spectral shift, nanoTAK – kinetic analysis in solution (kon up to 10⁸ M⁻¹s⁻¹, koff up to 2 s⁻¹)
- Materials
- Proteins, Membrane Proteins, Nucleic Acids/RNA, Antibodies/Biologics
- Certificates
- IQOQ Qualification
- Founded
- 2008
- Industries
- Pharma/Biotech, Drug Discovery, Biologics Development, Structural Biology, Targeted Protein Degradation, Academic Research
- 01
Research and development of measurement methods
- 02
Instrument development and manufacturing
- 03
Software and data analysis
- 04
Sales
- 05
Installation and IQOQ qualification
- 06
Service, maintenance and training
Sample Materials and Measurement Methods
Proteins
Primary sample material for stability and affinity measurements, also in crude lysates (Andromeda).
Membrane Proteins
Measurable in detergents, liposomes or nanodiscs without immobilization.
Nucleic Acids/RNA
Characterization of protein-nucleic acid and RNA-ligand interactions in solution.
Antibodies/Biologics
Developability and formulation profiling for biologics candidates.
Locations
Munich
DEHeadquarters, development and manufacturing
- Materials
- Proteins, membrane proteins, nucleic acids, biologics
- Prometheus Panta
- Dianthus / Dianthus uHTS
- Monolith Omni
- Andromeda
South San Francisco
USBranch office NanoTemper Technologies, Inc. (sales and support)
Product Lines
Inside the production
Industries
Processes & technical limits
| Processes | Tolerance | Max. part | Weight | Lot size | Materials |
|---|---|---|---|---|---|
| nanoDSF (nano Differential Scanning Fluorimetry) – protein stability characterizationTesting | – | – | – | – | Proteins, Biologics |
| Dynamic Light Scattering (DLS) – hydrodynamic radius, polydispersity, aggregationTesting | – | – | – | – | Proteins |
| Static Light Scattering (SLS) – molecular weight determination, self-interactionTesting | – | – | – | – | Proteins |
| Backreflection – turbidity/aggregate measurementTesting | – | – | – | – | Proteins |
| MicroScale Thermophoresis (MST) – binding affinity measurement in solutionTesting | – | – | – | – | Proteins, Nucleic acids, Small molecules |
| Temperature Related Intensity Change (TRIC) – fluorescence-based binding detectionTesting | – | – | – | – | Proteins |
| Spectral Shift – affinity determination via spectral shiftTesting | – | – | – | – | Proteins |
| nanoTAK – kinetic analysis in solution (kon up to 10⁸ M⁻¹s⁻¹, koff up to 2 s⁻¹)Testing | – | – | – | – | Proteins, Membrane proteins, Nucleic acids |
| High-Throughput Screening (Dianthus uHTS, 384-well plates)Testing | – | – | – | – | Proteins, Fragments, Compounds |
5 Anlagen & Maschinen
| Machine | Manufacturer / model | Quantity | Working area | Year built | Site |
|---|---|---|---|---|---|
| Protein Stability Measurement Instrument (nanoDSF/DLS/SLS/Backreflection) | NanoTemper Technologies Prometheus Panta | – | 10 µL sample volume | – | Munich |
| Affinity Screening Instrument (Spectral Shift/TRIC) | NanoTemper Technologies Dianthus | – | – | – | Munich |
| High-Throughput Screening Instrument | NanoTemper Technologies Dianthus uHTS | – | 384-well plates | – | Munich |
| MST Affinity/Kinetics Measurement Instrument | NanoTemper Technologies Monolith Omni | – | 10 µL sample volume | – | Munich |
| Protein Stability Measurement Instrument (crude lysates) | NanoTemper Technologies Andromeda | – | – | – | Munich |
Additional services
Quality
Documented IQOQ qualification (Installation Qualification / Operational Qualification) according to industry standards with signed forms for internal quality records; validation and relocation services complement quality assurance.
| Evidence | Munich |
|---|---|
| IQOQ Qualification |
History
- 2008
Founded as NanoTemper Technologies GmbH in Munich by Stefan Dühr and Philipp Baaske
- 2026
Launch of Monolith Omni and Dianthus α as well as new website