Miniaturized optical sensors and software for online biomass, oxygen and pH monitoring in shake flasks and bioreactors.
Scientific Bioprocessing Inc. (sbi)
Wilmington, DE (USA) · Pittsburgh, PA (USA) · Baesweiler (SBI Europe – aquila biolabs GmbH)New on Amplifa
About Scientific Bioprocessing
Scientific Bioprocessing Inc. (sbi), a subsidiary of Scientific Industries, Inc., develops and markets sensor technology and software for digital bioprocess development. The core product is the DOTS Platform ('Digitally Simplified Bioprocessing'): miniaturized smart sensors such as the Cell Growth Quantifier (CGQ) for non-invasive biomass measurement via backscatter, the Multiparameter Sensor (MPS) for DO, pH and fluorescence, optical DO Sensor Pills, the Liquid Injection System (LIS) for automated biomass-based fed-batch feeding, as well as the CGQ BioR biomass sensor and Flow Cells for pH and DO measurement in bioreactor flow loops. The systems are used for microbial fermentations and mammalian cell cultures in shake flasks and bioreactors and are controlled via the DOTS software with data acquisition and calibration (e.g. online OD600). Customers come from industry and academia, including Clariant, Bond Pet Foods as well as universities such as TU Wien, TU Dresden and RWTH Aachen. Locations are in Wilmington (DE) and Pittsburgh (PA) in the USA and in Baesweiler (SBI Europe, represented by aquila biolabs GmbH). The offering is complemented by installation, training and an Application Science Service for data analysis.
- Site
- 2 Mill Road, Suite 1100, Wilmington, DE 19806, USA
- Processes
- Biomass monitoring in shake flasks (CGQ, backscatter), Biomass monitoring in bioreactors (CGQ BioR), Online dissolved oxygen (DO) monitoring in shake flasks (DO Sensor Pills, optical), Multiparameter Sensor (MPS) for shake flasks (DO, pH, fluorescence), pH monitoring in flow loops (Flow Cells pH & DO), Continuous DO and pH monitoring in bioreactor flow loops (Flow Cells), Automated, biomass-based fed-batch liquid dosing in shake flasks (Liquid, Microbial Screening in shake flasks
- Materials
- Sulfolobus acidocaldarius, Pichia pastoris, Filamentous fungi, Microbial cultures, Mammalian cell cultures, Vienna Defined (VD) Medium, Cell Growth Quantifier (CGQ), Multiparameter Sensor (MPS)
- Industries
- Bioprocess Engineering, Biotechnology, Biomanufacturing, Pharmaceuticals
- 01
Sensor development (optical sensor technology)
- 02
Product development and software (DOTS)
- 03
Installation & Training (virtual and onsite)
- 04
Application Science Service (data analysis)
- 05
Maintenance & technical support
Applications & Organisms
Cell Growth Quantifier (CGQ)
Non-invasive, real-time biomass monitoring in shake flasks and bottles via backscatter light detection.
Multiparameter Sensor (MPS)
Online measurement of dissolved oxygen, pH and fluorescence in shake flasks.
DO Sensor Pills
Pill-based optical sensor for online monitoring of dissolved oxygen in shake flasks, also under baffled conditions.
Liquid Injection System (LIS)
Automated, parallelized and biomass-based fed-batch dosing of liquids (e.g. glucose) in shake flasks.
CGQ BioR
Real-time biomass monitoring in bioreactors, also under extreme conditions (e.g. 75–80 °C, pH 2–3, 400 h cultivation).
Flow Cells pH & DO
Continuous pH and DO monitoring in bioreactor flow loops via optical sensors.
DOTS Software
Data acquisition, calibration (e.g. online OD600) and control of shake flask and bioreactor processes.
Locations
Wilmington, DE (USA)
USNorth America location (2 Mill Road, Suite 1100)
Pittsburgh, PA (USA)
USNorth America location (201 Penn Center Blvd)
Baesweiler
DESBI Europe, represented by aquila biolabs GmbH (Arnold-Sommerfeld-Ring 2)
Products
Inside the production
Industries
Processes & technical limits
| Processes | Tolerance | Max. part | Weight | Lot size | Materials |
|---|---|---|---|---|---|
| Biomass monitoring in shake flasks (CGQ, backscatter)Testing | – | – | – | – | Microorganisms, Mammalian cells, Filamentous fungi |
| Biomass monitoring in bioreactors (CGQ BioR)Testing | – | – | – | – | Microorganisms (thermophilic up to 75-80 °C, pH 2-3), Mammalian cells |
| Online dissolved oxygen (DO) monitoring in shake flasks (DO Sensor Pills, optical)Testing | – | – | – | – | – |
| Multiparameter Sensor (MPS) for shake flasks (DO, pH, fluorescence)Testing | – | – | – | – | – |
| pH monitoring in flow loops (Flow Cells pH & DO)Testing | – | – | – | – | – |
| Continuous DO and pH monitoring in bioreactor flow loops (Flow Cells)Testing | – | – | – | – | – |
| Automated, biomass-based fed-batch liquid dosing in shake flasks (LiquidAssembly | – | – | – | – | Glucose solutions, Culture medium |
| Microbial Screening in shake flasksEngineering | – | – | – | – | Microorganisms |
| Biomass-based feeding (automated feeding depending on cell growth)Engineering | – | – | – | – | Microorganisms, Pichia pastoris |
| DOTS Software – data acquisition, calibration (e.g. online OD600) and process controlEngineering | – | – | – | – | – |
| Installation & Training (virtual and onsite)Assembly | – | – | – | – | – |
| Application Science Service – data analysis and consultingEngineering | – | – | – | – | – |
| Fluorescence MonitoringTesting | – | – | – | – | – |
7 Anlagen & Maschinen
| Machine | Manufacturer / model | Quantity | Working area | Year built | Site |
|---|---|---|---|---|---|
| Multiparameter Sensor (MPS) | Scientific Bioprocessing (sbi) MPS | – | Shake flasks | – | – |
| DO Sensor Pills (optical oxygen sensor) | Scientific Bioprocessing (sbi) DO Sensor Pill | – | Shake flasks | – | – |
| Liquid Injection System (LIS) | Scientific Bioprocessing (sbi) LIS | – | Shake flasks | – | – |
| Cell Growth Quantifier (CGQ) | Scientific Bioprocessing (sbi / aquila biolabs) CGQ | – | Shake flasks and bottles | – | – |
| Cell Growth Quantifier BioR (bioreactor biomass sensor) | Scientific Bioprocessing (sbi) CGQ BioR | – | Bioreactors (tested in a 3 L bioreactor with 2 L working volume) | – | – |
| Flow Cells pH & DO | Scientific Bioprocessing (sbi) Flow Cells | – | Bioreactor flow loops | – | – |
| DOTS Software | Scientific Bioprocessing (sbi) | – | – | – | – |
Additional services
Reference customers
Company History
- 2014
Founding of aquila biolabs by Jens Bayer, Daniel Grünes, Konrad Herzog and David Frank; introduction of the Cell Growth Quantifier (CGQ).
Founding of Fluorometrix Corp by Drs. Rao and Joe Qualitz based on patents for optical sensors (DO, pH, CO₂, glucose) – year not specified.
Scientific Industries (SI) acquires Fluorometrix and renames the company Scientific Bioprocessing Inc. (sbi).
sbi and aquila biolabs pool their strengths and introduce the DOTS Platform (Digitally Simplified Bioprocessing).